BACTERIA STORAGE AND CONSERVATION

CRYOBANK™ is based on a cryovial system comprising chemically treated beads covered with a special cryogenic preserving solution.

CRYOBANK™ offers a reliable, convenient and versatile system for storing and preserving fastidious bacteria over long period.

CRYOBANK™ is less expansive than lyophilisation, repeated subculture or purchasing commercially available control organisms. It permits the easy establishment of culture collections for laboratory accreditation or research purposes.

CRYOBANK™ is suitable for use in all laboratories dealing with bacteria including:
clinical, water, brewing, veterinary, food, pharmaceutical, dairy, biotechnology...

> Cryobank Red (64 cryovials)
> Cryobank Blue (64 ccryovials)
> Cryobank Yellow (64 cryovials)
> Cryobank Green (64 cryovials)
> Cryobank Mixed colors(64 cryovials)
> Cryoblock (1 cryoblock)

SIMPLE TO USE PROCEDURE: Storage of the organism

1) Using a permanent marker, label the code for the organism onto the white panel on the vial. CRYOBANK tube with colonies from a fresh, pure culture to a density equivalent to MacFarland 3 or 4 standard.

2) Replace cap and mix carefully by inverting the tube to completely distribute the organism..

3) Remove as much of the cryopreservative fluid as possible with a sterile pipette and re-close the tube..

4) Store the inoculated CRYOBANK tube in a suitable freezer. Details of the tube contents may also be recorded on the grid printed on the lid of the storage box. Space is also available for recording the individual box identification if required.

SIMPLE TO USE PROCEDURE: Recovery of the organism

5) Remove the CRYOBANK tube from the freezer. If several tubes are being removed at once, thawing should be prevented by use of a cryoblock. Open the vial and remove a bead by inserting a sterile needle through the hole or by using sterile forceps.

6) The bead may then be dropped into an appropriate liquid medium.

7) Alternatively, streak the bead immediately over the surface of an appropriate solid medium and incubate under conditions.

8) Discard the bead safely in the recommended manner for infected material. Close the vial and return it to the freezer as soon as possible.